Welcome to our Resources Hub


Find here all our reference documents to better understand our tests, their regulatory context and their applications. Resources mainly designed for R&D, regulatory affairs, toxicology and ecotoxicology teams.

Brochures and technical documents

Our sector-specific brochures give you a clear picture of what Watchfrog tests concretely bring to your business: regulatory context, endocrine axes covered, and positioning relative to your sector's requirements.

Our technical documents go further: for each test, you'll find information on the protocol, the biological principle, result reading criteria and associated scientific references.

Publications (protected access with request form)

Our doctors regularly publish articles in partnership with industry players and other scientists, with the aim of advancing science as well as regulations at a global level.

A selection of publications is available here upon request.

The Xenopus Eleutheroembryo Thyroid Assay (XETA), a New Approach Methodology (NAM) for the regulatory assessment of thyroid activity of chemicals.

Du Pasquier David, Tindall Andrew, Weltje Lennart, Lagadic Laurent, Lemkine Gregory.

Environ Toxicol Chem. 2025

Testing the sensitivity of the medaka Transgenic Eleuthero-embryonic THYroid- Specific assay (TETHYS) to different mechanisms of action.

Elise Pesce, Jessica Benitez-Gonzalez, Andrew J Tindall, Gregory F Lemkine, Barbara
Robin-Duchesne, Laurent M Sachs, David Du Pasquier.

Aquat Toxicol. 2024;276:107081.

Electrochemical degradation of diclofenac generates unexpected thyroidogenic transformation products: Implications for environmental risk assessment.

Rafael Reis, Rebecca Dhawle, Romain Girard, Zacharias Frontistis, Dionissios Mantzavinos, Peter de Witte, Deirdre Cabooter, David Du Pasquier.

J Hazard Mater. 2024; 472:134458.

How the Xenopus eleutheroembryonic thyroid assay compares to the amphibian metamorphosis assay for detecting thyroid active chemicals

David Du Pasquier, Benoît Salinier, Katherine K. Coady, Alan Jones, Oliver Körner, Jessica LaRocca, Gregory Lemkine, Barbara Robin-Duchesne, Lennart Weltje, James R. Wheeler, Laurent Lagadic

Regulatory Toxicology and Pharmacology 149 (2024) 105619

Overlapping action of T3 and T4 during Xenopus laevis development

Alicia Tribondeau, David Du Pasquier, Médine Benchouaia, Corinne Blugeon, Nicolas Buisine and Laurent M. Sachs (2024)

Frontiers in Endocrinology 10.3389/fendo.2024.1360188

Evaluation of the endocrine activity of surface water samples using aquatic eleuthero-embryos—A comparison with in vitro assays

Tindall, A. J., Du Pasquier, D., & Lemkine, G. F. (2023)

Water Environment Research, 95(8), e10911.

A Novel Transgenic Model to Study Thyroid Axis Activity in Early Life Stage Medaka

Elise Pesce, Marion Garde, Muriel Rigolet, Andrew J. Tindall, Gregory F. Lemkine, Lisa A. Baumann, Laurent M. Sachs, and David Du Pasquier

Environmental Science & Technology (2023).

Electrochemical degradation of 17α-ethinylestradiol: Transformation products, degradation pathways and in vivo assessment of estrogenic activity

Rafael Reis, Rebecca Dhawle, David Du Pasquier, Andrew J. Tindall, Zacharias Frontistis, Dionissios Mantzavinos, Peter de Witte, Deirdre Cabooter

Environment International 176 (2023) 107992

Oestrogen reporter transgenic medaka for non-invasive evaluation of aromatase activity

Petra Spirhanzlova, Mathilde Leleu, Anthony Sébillot, Gregory F. Lemkine, Taisen Iguchi, Barbara A. Demeneix, Andrew J. Tindall

Comparative Biochemistry and Physiology, Part C 179 (2016) 64–71

Rapid Fluorescent Detection of (Anti)androgens with spiggin-gfp Medaka

Anthony Sébillot, Pauliina Damdimopoulou, Yukiko Ogino, Petra Spirhanzlova, Shinichi Miyagawa, David Du Pasquier, Nora Mouatassim, Taisen Iguchi, Gregory F. Lemkine, Barbara A. Demeneix and Andrew J. Tindall

Environmental Science & Technology (2014)

Posters (protected access with request form)

Watchfrog teams take part in key scientific events.

Find here, upon request, the posters presented by our doctors at these events.

Assessing Wastewater and Sewage Sludge Treatment Efficiency by Combining Physicochemical Characterization and in vivo Endocrine Disruption Bioassays

Medaka and Zebrafish Display Contrasting Responses to Chronic Exposure to Ethinyl Estradiol

Bridging In Vivo and In Vitro Evidence from New Approach Methodologies (NAMs): Comparing XETA and ToxCast Data to Identify Thyroid-Active Substances

Screening ANSES priority substances for thyroid activity using two Eleuthero-embryonic Bioassays