Fluorescent-based screening in translucent amphibian models:
the new generation of WatchFrog tests "light up" to provide biological answers.
In vivo information...
- integrated approach: taking into account the complete metabolism of the compound
- amphibian model: main organs system like those of mammals and humans
- in particular for the endocrine, angiogenic, immune and neurogenic systems
- simultaneous poly-detection of multiple biological markers
... from in vitro tools:
- simplicity of administering liquid sample by direct contact with the larvae
- compatibility with standard equipment
- small quantity of active substance needed: tests in micro-wells
- high statistical validity: each set of eggs giving approx. 2000 individual tests
- rapidity of screening: fluorescence detectable after a few hours
- ease of reading results by measuring fluorescence via transparency
- ease of quantification
- minimization of ethical issues by virtue of using amphibian larvae
- low cost of amphibian maintenance and production.